| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | COX4I1 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
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| Catalog no. (Mfr.) | |
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Product Overview
U-Blot® COX IV Mouse Monoclonal Antibody (6C8), AbFluor 594 Conjugated is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against COX IV. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Rat, Mouse.
Validated Applications
| Target | COX IV |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 6C8 |
| Isotype | IgG1 |
| Conjugate | AbFluor 594 |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | COX IV Monoclonal Antibody(6C8) AbFluor™ 594 Conjugated specially designed for your Immunofluorescence analysis. |
| UniProt / Swiss-Prot | P13073 (Human) |
Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:50-300;IF 1:200 .
Immunogen / Antigen
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:50-300;IF 1:200 .
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: AbFluor 594.
Store as follows: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Clone 6C8.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
