| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | FTO;KIAA1752;Alpha-ketoglutarate-dependent dioxygenase FTO;Fat mass and obesity-associated protein |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® FTO Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against FTO. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | FTO |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0383R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 58 kDa | Observed: 58 kDa |
| UniProt / Swiss-Prot | Q9C0B1 (Human) | Q8BGW1 (Mouse) | Q2A121 (Rat) |
Recommended dilutions: IHC 1:3000-1:20000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Alpha-ketoglutarate-dependent dioxygenase FTO
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:3000-1:20000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0383R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
