| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | HIST1H3A |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Histone H3 Mouse Monoclonal Antibody, FITC Conjugated is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Histone H3. It has been validated for Western Blot and reacts with Zebrafish.
Validated Applications
| Target | Histone H3 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugate | FITC |
| Reactivity | Zebrafish |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | Histone H3 Monoclonal Antibody FITC conjugated specially designed for your WB or IHC analysis. |
| UniProt / Swiss-Prot | P68431;Q71DI3;P84243 (Human) |
Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator
Immunogen / Antigen
Histone H3.1/Histone H3.2/Histone H3.3
Reactivity Notes
Confirmed cross-reactivity: Zebrafish. Confirm suitability for untested species before use.
Scientific Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator
Confirmed reactivity: Zebrafish. Other species should be validated by the user.
Conjugate: FITC.
Store as follows: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.