| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human p65 around the mono-methylation site of K314/K315. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® NFκB-p65 (Mono Methyl Lys314/Lys315) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NFkB p65. Recognizes Mono Methyl. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | NFkB p65 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Mono-Methyl-NFκB-p65 (K314/K315) Polyclonal Antibody detects endogenous levels of p65 protein only when mono-methylated at K314/K315. |
| Molecular Weight | Observed: 60 kDa |
| UniProt / Swiss-Prot | Q04206 (Human) | Q04207 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
Immunogen / Antigen
Synthesized peptide derived from human p65 around the mono-methylation site of K314/K315.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
