| Field | Specification |
|---|---|
| Alternative Names | Kmea |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Pan-Methacryllysine Mouse mAb is a recombinant monoclonal antibody raised in Mouse that specifically recognizes Methacryloyllysine (Lysine methacryloylation). It is designed for detection and analysis of the Lysine methacryloylation post-translational modification across research applications.
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Histone lysine methacrylation (Kmea) is a novel post-translational modification of proteins. It is a structural isomer of crotonylation, but they differ in mechanism and function. Specifically, methacrylate is the metabolic precursor of Kmea, and HAT1, SIRT1 and SIRT2 are the writer and eraser of Kmea respectively. In addition, 27 histone Kmea sites were identified in HeLa cells. Leigh syndrome is a neurological disease characterized by mitochondrial defects. Due to the genetic mutations of ECHS1 and Hibch enzymes in the metabolic approach of pyrine, causing the accumulation of methacrylyl-coA in patients with these mutations. The discovery of Kmea suggests a new direction for the pathological role of methacrylyl-coA accumulation.
Validated Applications
| Application | Western Blot, Immunohistochemistry |
|---|---|
| Recommended Dilution | WB 1:500–1:1000, IHC-P 1:500–1:2000 |
| Host | Mouse |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Protein G and immunogen affinity purified |
| Molecular Weight | Calculated: Multiple | Observed: Multiple |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse, Rat. It targets the Lysine methacryloylation modification and is suitable for Western Blot, Immunohistochemistry.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500–1:1000, IHC-P 1:500–1:2000.
Use WB 1:500–1:1000, IHC-P 1:500–1:2000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Methacryloyllysine (Lysine methacryloylation); reactivity: Human, Mouse, Rat.
The recombinant monoclonal antibody (Mouse host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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