| Field | Specification |
|---|---|
| Target | |
| Alternative names | pThr |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | polypeptide |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Pan-Phospho-Threonine Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-Threonine (Threonine phosphorylation [Thr(T)]). It is designed for detection and analysis of the Threonine phosphorylation [Thr(T)] post-translational modification across research applications.
Histones and non-histone proteins undergo diverse enzyme-catalyzed post-translational modifications, including phosphorylation, acetylation, methylation and ubiquitination. Threonine phosphorylation is one of the most conserved and essential reversible protein modifications in eukaryotes, catalyzed by protein kinases and reversed by protein phosphatases.It widely exists in cell signaling molecules, transcription factors, histones and structural proteins. Threonine phosphorylation participates in regulating cell cycle progression, cell proliferation, differentiation, apoptosis, metabolic homeostasis and DNA damage response. Abnormal threonine phosphorylation levels are closely associated with tumorigenesis, inflammatory disorders, autoimmune diseases and metabolic dysfunction.
Validated Applications
| Application | Western Blot, Immunofluorescence |
|---|---|
| Recommended Dilution | WB 1:500-1000, ICC/IF 1:50 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: Multiple | Observed: Multiple |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse, Rat. It targets the Threonine phosphorylation [Thr(T)] modification and is suitable for Western Blot, Immunofluorescence.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:500-1000, ICC/IF 1:50.
Use WB 1:500-1000, ICC/IF 1:50. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-Threonine (Threonine phosphorylation [Thr(T)]); reactivity: Human, Mouse, Rat.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
