| Field | Specification |
|---|---|
| Alternative Names | Kme3 |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Methylated KLH conjugates |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Pan-Tri-Methyllysine Rabbit pAb (KLH-conjugate immunogen) is a polyclonal antibody raised in Rabbit that specifically recognizes Tri-Methyllysine (Lysine trimethylation [Lys(K)]). It is designed for detection and analysis of the Lysine trimethylation [Lys(K)] post-translational modification across research applications.
Histones undergo a wide range of enzyme-catalyzed post-translational modifications, including acetylation, methylation, phosphorylation and ubiquitination. Lysine trimethylation is a vital epigenetic modification that predominantly occurs on histone lysine residues. It is dynamically regulated by histone methyltransferases and demethylases, and extensively distributed in eukaryotic chromatin.Lysine trimethylation participates in governing chromatin structure remodeling, transcriptional activation or repression, cell cycle regulation, and DNA damage repair. Abnormal trimethylation levels are closely associated with tumorigenesis, developmental disorders, inflammation and neurodegenerative diseases, acting as a pivotal molecular marker for epigenetic research and disease mechanism investigation.
Validated Applications
| Application | ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunoaffinity Chromatography |
|---|---|
| Recommended Dilution | ELISA 1:2000, WB 1:2000, IP 5 ug/mg protein sample, IF 5 ug/mL or 1:100 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Reactivity | Species Independent |
| Conjugate | Unconjugated |
| Purification | N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose |
| Molecular Weight | Calculated: Multiple | Observed: Multiple |
Immunogen / Antigen
Methylated KLH conjugates
Reactivity Notes
This antibody shows reactivity with Species Independent. It targets the Lysine trimethylation [Lys(K)] modification and is suitable for ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunoaffinity Chromatography.
Safety & Handling
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Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunoaffinity Chromatography. Recommended dilutions: ELISA 1:2000, WB 1:2000, IP 5 ug/mg protein sample, IF 5 ug/mL or 1:100.
Use ELISA 1:2000, WB 1:2000, IP 5 ug/mg protein sample, IF 5 ug/mL or 1:100. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Tri-Methyllysine (Lysine trimethylation [Lys(K)]); reactivity: Species Independent.
The polyclonal antibody (Rabbit host) was raised against Methylated KLH conjugates.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Product is stable for several weeks at 4°C. For extended storage, store product at -20ºC. | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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