| Field | Specification |
|---|---|
| Alternative Names | TNNC1, TNNI3 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-Cardiac Troponin I (Ser23/24) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-Cardiac Troponin I (Ser23/24) (Phosphorylation [Ser23/24]). It is designed for detection and analysis of the Phosphorylation [Ser23/24] post-translational modification across research applications.
Cardiac troponin is composed of three subunits, namely cardiac troponin T (cTnT), cardiac troponin I (cTnI), and cardiac troponin C (cTnC). They collectively participate in the regulation of cardiac muscle contraction. The amino-terminal and carboxyl-terminal ends of cTnI molecules are prone to degradation through protein hydrolysis. Cardiac troponin I (cTnI) is an important regulatory factor that inhibits muscle contraction under normal circumstances. It binds to actin, preventing the interaction between actin and cardiac troponin C (cTnC), thus inhibiting muscle contraction. The phosphorylation status of cTnI can alter its affinity for actin, thereby influencing the regulation of muscle contraction. The serine residues at positions 22 and 23 of cTnI are susceptible to phosphorylation by protein kinase A, which regulates muscle contraction. Abnormal levels of phosphorylation may also contribute to muscle dysfunction or the development of heart disease. Cellular localization: cardiac myofibril, cytosol.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:5000-1:10000 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 24 kDa | Observed: 25 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Ser23/24] modification and is suitable for Western Blot.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot. Recommended dilutions: WB 1:5000-1:10000.
Use WB 1:5000-1:10000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-Cardiac Troponin I (Ser23/24) (Phosphorylation [Ser23/24]); reactivity: Human.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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