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| Immunogen | polypeptide |
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Product Overview
U-Blot® Phospho-HDAC4(Ser632)/HDAC5(Ser661)/HDAC7(Ser486) Rabbit pAb is a polyclonal antibody raised in Rabbit that specifically recognizes Phospho-HDAC4 (Ser632)/HDAC5 (Ser661)/HDAC7 (Ser486) (Phosphorylation [HDAC4 Ser632, HDAC5 Ser661, HDAC7 Ser486]). It is designed for detection and analysis of the Phosphorylation [HDAC4 Ser632, HDAC5 Ser661, HDAC7 Ser486] post-translational modification across research applications.
Histone deacetylase 4, also known as HDAC4, is a protein that is encoded by the HDAC4 gene in human. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. This protein does not bind DNA directly but through transcription factors MEF2C and MEF2D. Furthermore, HDAC4 is required for TGFbeta1-induced myofibroblastic differentiation. In addition, the highly conserved HDAC4 Ser632, HDAC5 Ser498 (Ser661) and HDAC7 Ser486 residues are all phosphorylated by CAMK and PKD kinases in response to multiple cell stimuli, including VEGF-induced angiogenesis in endothelial cells, B cell and T cell activation, and differentiation of myoblasts into muscle fiber.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:1000-1:2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 140 kDa / 140 kDa / 100 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse. It targets the Phosphorylation [HDAC4 Ser632, HDAC5 Ser661, HDAC7 Ser486] modification and is suitable for Western Blot.
Safety & Handling
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It is validated for Western Blot. Recommended dilutions: WB 1:1000-1:2000.
Use WB 1:1000-1:2000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-HDAC4 (Ser632)/HDAC5 (Ser661)/HDAC7 (Ser486) (Phosphorylation [HDAC4 Ser632, HDAC5 Ser661, HDAC7 Ser486]); reactivity: Human, Mouse.
The polyclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
