| Field | Specification |
|---|---|
| Alternative Names | Heat shock protein beta-1, HspB1, SRP27 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-HSP27 (Ser78) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-HSP27 (Ser78) (Phosphorylation [Ser78]). It is designed for detection and analysis of the Phosphorylation [Ser78] post-translational modification across research applications.
Hsp27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers. It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization
Validated Applications
| Application | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
|---|---|
| Recommended Dilution | WB 1:500-1:1000, IHC-P 1:100-1:500, ICC/IF 1:50, IP 1:50-1:100 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG/Kappa |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 23 kDa | Observed: 27 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Ser78] modification and is suitable for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
Safety & Handling
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It is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation. Recommended dilutions: WB 1:500-1:1000, IHC-P 1:100-1:500, ICC/IF 1:50, IP 1:50-1:100.
Use WB 1:500-1:1000, IHC-P 1:100-1:500, ICC/IF 1:50, IP 1:50-1:100. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-HSP27 (Ser78) (Phosphorylation [Ser78]); reactivity: Human.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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