| Field | Specification |
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| Alternative names | BM28, CCNL1, CDCL1, KIAA0030 |
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| Applications | |
| Immunogen | polypeptide |
| Conjugate | |
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Product Overview
U-Blot® Phospho-MCM2 (Ser139) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-MCM2 (Ser139) (Phosphorylation [Ser139]). It is designed for detection and analysis of the Phosphorylation [Ser139] post-translational modification across research applications.
The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork. This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur. Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis. CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading in vitro. Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication. MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest.
Validated Applications
| Application | Western Blot, Immunohistochemistry, Immunoprecipitation |
|---|---|
| Recommended Dilution | WB 1:1000, IHC-P 1:1000, IP 1:100 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 102 kDa | Observed: 102 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Ser139] modification and is suitable for Western Blot, Immunohistochemistry, Immunoprecipitation.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot, Immunohistochemistry, Immunoprecipitation. Recommended dilutions: WB 1:1000, IHC-P 1:1000, IP 1:100.
Use WB 1:1000, IHC-P 1:1000, IP 1:100. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-MCM2 (Ser139) (Phosphorylation [Ser139]); reactivity: Human.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
