| Field | Specification |
|---|---|
| Alternative Names | MAP2K1, PRKMK1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-MEK1 (Ser298) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-MEK1 (Ser298) (Phosphorylation [Ser298]). It is designed for detection and analysis of the Phosphorylation [Ser298] post-translational modification across research applications.
MEK1 and MEK2, also known as MAPK or Erk kinases, are dual-specificity protein kinases. Their primary role is to phosphorylate and activate downstream MAPK proteins, including ERK1/2 (Extracellular Signal-Regulated Kinase 1/2). Upon stimulation by growth factors, hormones, or stress, MEK1 undergoes activation and phosphorylation. This activated form of MEK1 then transmits signals to ERK1/2 through phosphorylation, ultimately leading to their activation. Once activated, ERK1/2 can enter the cell nucleus and play a crucial role in regulating various cellular physiological processes, such as gene expression, cell cycle progression, cell proliferation, cell migration, and cell survival. In typical conditions, MEK1 is phosphorylated at two conserved serine residues, namely Ser218 and Ser222. Additionally, MEK1 can also be phosphorylated at the Ser298 site by pAK1. This phosphorylation event helps facilitate signal transduction from Raf to MEK1 and Erk2.
Validated Applications
| Application | Western Blot, Immunofluorescence |
|---|---|
| Recommended Dilution | WB 1:1000, ICC/IF 1:100 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 43 kDa | Observed: 42 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse. It targets the Phosphorylation [Ser298] modification and is suitable for Western Blot, Immunofluorescence.
Safety & Handling
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It is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000, ICC/IF 1:100.
Use WB 1:1000, ICC/IF 1:100. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-MEK1 (Ser298) (Phosphorylation [Ser298]); reactivity: Human, Mouse.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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