| Field | Specification |
|---|---|
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb is a recombinant monoclonal antibody raised in Mouse that specifically recognizes Phospho-Myosin Light Chain 2 (Ser19) (Phosphorylation [Ser19]). It is designed for detection and analysis of the Phosphorylation [Ser19] post-translational modification across research applications.
Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers. Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity. Phosphorylation by cdc2 controls the timing of cytokinesis.
Validated Applications
| Application | Western Blot, Immunofluorescence |
|---|---|
| Recommended Dilution | WB 1:1000, ICC/IF 1:50 |
| Host | Mouse |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG1/Kappa |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 20 kDa | Observed: 18 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Ser19] modification and is suitable for Western Blot, Immunofluorescence.
Safety & Handling
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It is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000, ICC/IF 1:50.
Use WB 1:1000, ICC/IF 1:50. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-Myosin Light Chain 2 (Ser19) (Phosphorylation [Ser19]); reactivity: Human.
The recombinant monoclonal antibody (Mouse host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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