| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | B23, numatrin, NO38 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | polypeptide |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Phospho-NPM1 (Thr199) Rabbit pAb is a polyclonal antibody raised in Rabbit that specifically recognizes Phospho-NPM1 (Thr199) (Phosphorylation [Thr199]). It is designed for detection and analysis of the Phosphorylation [Thr199] post-translational modification across research applications.
Nucleophosmin (NPM), also known as B23, numatrin or NO38, is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase. NPM associates with unduplicated centrosomes and is a direct substrate of Cdk2-cyclin E in centrosome duplication. Upon phosphorylation at Thr199 by Cdk2-cyclin E, NPM dissociates from centrosomes, and this dissociation is a prerequisite step for centrosome to initiate duplication.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:1000-1:2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 33 kDa | Observed: 38 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse. It targets the Phosphorylation [Thr199] modification and is suitable for Western Blot.
Safety & Handling
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It is validated for Western Blot. Recommended dilutions: WB 1:1000-1:2000.
Use WB 1:1000-1:2000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-NPM1 (Thr199) (Phosphorylation [Thr199]); reactivity: Human, Mouse.
The polyclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
