| Field | Specification |
|---|---|
| Alternative Names | p21-activated kinase 1, PAK1T212ph |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-PAK1 (Thr212) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-PAK1 (Thr212) (Phosphorylation [Thr212]). It is designed for detection and analysis of the Phosphorylation [Thr212] post-translational modification across research applications.
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth. Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK. Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1. Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1, and Ser192 and Ser197 of PAK2. Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation. Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity. Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck. PAK4, PAK5/7, and PAK6 have lower sequence similarity with PAK1-3 in the amino terminal regulatory region. Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4. PAK family members are widely expressed, and often overexpressed in human cancer.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:1000-1:5000 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 61 kDa | Observed: 62 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human, Mouse. It targets the Phosphorylation [Thr212] modification and is suitable for Western Blot.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot. Recommended dilutions: WB 1:1000-1:5000.
Use WB 1:1000-1:5000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-PAK1 (Thr212) (Phosphorylation [Thr212]); reactivity: Human, Mouse.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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