| Field | Specification |
|---|---|
| Alternative Names | SIR2L1, hSIRT1, hSIR2, 75SirT1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Phospho-SIRT1(Ser47) Rabbit mAb is a recombinant monoclonal antibody raised in Rabbit that specifically recognizes Phospho-SIRT1 (Ser47) (Phosphorylation [Ser47]). It is designed for detection and analysis of the Phosphorylation [Ser47] post-translational modification across research applications.
SIRT1 is a member of the Silent Information Regulator2 (SIR2) family, which is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined.
Validated Applications
| Application | Western Blot, Immunohistochemistry |
|---|---|
| Recommended Dilution | WB 1:500-1:1000, IHC-P 1:50-1:200 |
| Host | Rabbit |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG/Kappa |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 82 kDa | Observed: 110 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Ser47] modification and is suitable for Western Blot, Immunohistochemistry.
Safety & Handling
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It is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-1:1000, IHC-P 1:50-1:200.
Use WB 1:500-1:1000, IHC-P 1:50-1:200. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-SIRT1 (Ser47) (Phosphorylation [Ser47]); reactivity: Human.
The recombinant monoclonal antibody (Rabbit host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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