| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Ube1L / UBA7 was used as the immunogen for the UBA7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
UBA7 Antibody / Ubiquitin like modifier activating enzyme 7 / Ube1L is a anti-UBA7 Rabbit antibody Recombinant Rabbit Monoclonal clone 31U08 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: UBA7
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31U08, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, FACS
Biological background
UBA7 antibody is widely applied in immunology, virology, and cancer biology. UBA7 is induced by type I interferons and mediates host defense against viral infection. ISGylation of viral and host proteins disrupts replication cycles and enhances antiviral resistance. By detecting UBA7, researchers can investigate how interferon pathways regulate protein modification and innate immunity.
Western blot assays detect UBA7 protein in interferon treated cells, while immunohistochemistry maps its expression in immune tissues. Immunofluorescence highlights cytoplasmic and nuclear localization, consistent with its role in protein conjugation. These applications make UBA7 antibody a versatile tool for studying antiviral responses and immune regulation.
Beyond virology, UBA7 contributes to tumor suppression. Loss of UBA7 expression has been linked to lung cancer and leukemia, where reduced ISGylation disrupts normal cell regulation. UBA7 also interacts with ubiquitin conjugating enzymes, linking ISGylation to broader protein quality control mechanisms. By applying UBA7 antibody, scientists can evaluate how ubiquitin like modifications influence cancer biology and immune defense.
UBA7 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
