| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human HSPC150 was used as the immunogen for the UBE2T antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
UBE2T Antibody / HSPC150 is a anti-UBE2T Rabbit antibody Recombinant Rabbit Monoclonal clone 31U48 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: UBE2T
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31U48, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Ubiquitin-conjugating enzyme E2 T functions by transferring activated ubiquitin from the E1 enzyme to E3 ligases, which then attach ubiquitin to substrates. Research using UBE2T antibody has shown that this enzyme is critical for DNA crosslink repair. Monoubiquitination of FANCD2-FANCI is a central step in activating the Fanconi anemia pathway, allowing recruitment of downstream repair proteins to stalled replication forks. Without UBE2T activity, this repair process fails, resulting in genomic instability and increased sensitivity to DNA crosslinking agents.
Mutations in UBE2T cause a subtype of Fanconi anemia, a genetic disorder characterized by bone marrow failure, developmental abnormalities, and cancer predisposition. Studies with UBE2T antibody have revealed that patient-derived mutations impair the enzyme's ability to transfer ubiquitin, leading to defective DNA repair. Because of this essential function, UBE2T has emerged as a critical component of genome maintenance.
Beyond inherited disease, UBE2T is implicated in cancer. Research using UBE2T antibody has demonstrated that the protein is frequently overexpressed in tumors such as breast, lung, and prostate cancers. Elevated levels enhance DNA repair capacity and promote resistance to chemotherapy. UBE2T overexpression also correlates with tumor aggressiveness, making it a potential prognostic biomarker and therapeutic target. Inhibiting UBE2T could sensitize cancer cells to DNA damaging therapies.
UBE2T antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein levels across tissues, immunohistochemistry reveals expression patterns in tumors, and immunofluorescence localizes UBE2T in nuclei of proliferative cells. These applications make UBE2T antibody valuable for research into DNA repair and cancer biology.
By providing validated UBE2T antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
