| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
UltraNuclease is a genetically engineered endonuclease from Serratia marcescens. The enzyme digests all forms of DNA/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.
UltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.
Features
- Wide range of applications: degrade all forms of DNA and RNA
- High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×106 U/mg
- Strong adaptability: strong tolerance, adapt to a variety of reaction conditions
- Comply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin
- Qualification: product gets the FDA DMF filling which can shorten the customers for new drug application
- GMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture
Application
- Purification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA/RNA from proteins and other biologicals
- Reduction of viscosity caused by nucleic acids
- Sample preparation in electrophoresis and chromatography
- Prevention of cell clumping
Specification
| Expression Host | Recombinant E. coli with UltraNuclease gene |
| Molecular Weight | 26.5 kDa |
| Isoelectric point | 6.85 |
| Purity | ≥ 99% (SDS-PAGE) |
| Storage Buffer | 20 mM Tris-HCl pH8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin |
| Unit Definition | The definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides). |
Components
| Components No. | Name | 20157ES25 (25 KU) | 20157ES60 (100 KU) | 20157ES80 (1 MU) | 20157ES90 (5 MU) |
| 20157 | UltraNuclease GMP-grade (250 U/μL) | 100 μL | 400 μL | 4 mL | 20 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.
Figures
- Quality
|
No. |
Test Items |
Competitor |
Yeasen |
|
1 |
Source |
E. coli |
E. coli |
|
2 |
Activity |
≥ 250 units/μL |
250-300 units/μL |
|
3 |
Specific Activity |
≥1.1×106 U/mg |
≥1.5×106 U/mg |
|
4 |
Purity |
≥ 99%（SDS） |
≥ 99%（HPLC） |
|
5 |
Endotoxin |
< 0.25EU/1000U |
< 0.25EU/1000U |
|
6 |
Protease |
None Detected |
None Detected |
- UltraNuclease Enzymatic Activity Under Different Conditions
Temperature & pH
Figure 1. Effects of temperature and pH on enzyme activity.
(A) Enzyme activity increases with temperature, with the optimum at 37 °C.
(B) The enzyme is active within pH 4–10, with pH 8.0 as the optimum.
Salt ion concentration
Figure 2. Effects of salt ion concentration on enzyme activity.
(A). Na⁺/K⁺ inhibit enzyme activity; activity is completely lost above 300 mM, while good activity is retained below 20 mM.
(B). Maximum activity is observed with 5 mM Mg²⁺; in the absence of Mg²⁺, 1–2 mM Mn²⁺ can support activity.
(C). Increasing PO₄³⁻ concentration significantly inhibits enzyme activity.
Surfactants
Figure 3. Effects of surfactants on enzyme activity.
(A) Triton® X-100 and (B) sodium deoxycholate reduce enzyme activity and should be maintained at low concentrations.
Protein denaturants
Figure 4. Effect of protein denaturants on enzyme activity.
SDS and urea reduce enzyme activity and should be maintained at low concentrations.
Other commonly used reagents
Figure 5. Effects of different inhibitors on UltraNuclease activity.
(A). Enzyme activity is strongly influenced by ammonium sulfate concentration.
(B). PMSF shows little effect on enzyme activity.
(C). At low EDTA concentrations (≤0.5 mM), enzyme activity is largely unaffected, whereas at 5 mM EDTA, activity is completely inhibited.
- Robust Performance
Figure 6. Single-factor test comparison with Supplier M*.
No significant difference was observed between Yeasen UltraNuclease and supplier M*.
Strong stability
Figure 7. Stability of UltraNuclease.
(A) Enzyme activity remained unchanged after 4 weeks of storage at –20 °C and 4 °C, and after 2 weeks at 37 °C.
(B) Enzyme activity was unaffected after 10 freeze–thaw cycles between –80 °C and –25 °C.
- Reaction conditions recommend
Table 1. The optimal reaction conditions for UCF.ME™ UltraNuclease
| Factor | Optimum Condition | Effective condition |
| Mg2+ | 1-5mM | 0-10mM |
| pH | 8-9 | 6-10 |
| Temperature | 37℃ | 0-42℃ |
| Na+/K+ | 0-20mM | 0-150mM |
| PO43+ | 0-10mM | 0-100mM |
| EDTA | 0-0.5mM | 0-5mM |
| Triton®X-100 | 0%-0.4% | 0%-1% |
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Nucleic Acid Purification applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme that degrades 1 µg of DNA or RNA substrate to acid-soluble form in 30 min at 37°C under defined buffer conditions.
This enzyme is produced as Recombinant (E. coli) and is available in GMP grade for regulated applications. This enzyme is manufactured in an ISO 13485-certified UCF.ME™ facility and meets GMP-grade quality standards. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Nuclease activity typically requires divalent metal ion cofactors (Mg²⁺ or Mn²⁺ at 1–5 mM). Activity is inhibited by chelating agents such as EDTA (≥1 mM), high salt concentrations, and reducing agents such as DTT at elevated concentrations. Heat inactivation (65–75°C for 15 min) is effective for most nucleases, allowing removal of enzyme activity after digestion without column purification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
