| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human UFD1L was used as the immunogen for the UFD1L antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
UFD1L Antibody / Ubiquitin Fusion Degradation 1 Like is a anti-UFD1L Rabbit antibody Recombinant Rabbit Monoclonal clone 29U95 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: UFD1L
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29U95, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, FACS
Biological background
The UFD1L-NPL4-p97 complex functions as a segregase, using ATP hydrolysis to unfold and extract ubiquitinated proteins for degradation by the proteasome. Research using UFD1L antibody has demonstrated that this complex acts in multiple pathways, including clearance of misfolded proteins from the endoplasmic reticulum, disassembly of protein aggregates, and regulation of spindle assembly during mitosis. Its versatile roles make UFD1L a central hub in protein quality control networks.
Mutations and deletions involving UFD1L are associated with genetic disorders. Studies with UFD1L antibody have shown that haploinsufficiency contributes to DiGeorge syndrome, a developmental disorder characterized by congenital heart defects, immune dysfunction, and craniofacial anomalies. Because UFD1L is located in the 22q11 region, deletions that remove this gene disrupt proteostasis during development, contributing to disease phenotypes.
In cancer biology, UFD1L has been implicated in regulating cell proliferation and survival. Research using UFD1L antibody has revealed that overexpression enhances proteasomal degradation of regulatory proteins, supporting uncontrolled growth. Conversely, loss of function can cause accumulation of toxic aggregates, impairing viability. These dual roles highlight its importance in maintaining balanced proteostasis across different contexts.
UFD1L antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting identifies endogenous levels, immunohistochemistry localizes expression in developmental tissues, and immunoprecipitation demonstrates interactions with p97/VCP and NPL4. These applications make UFD1L antibody essential in both developmental and disease research.
By supplying validated UFD1L antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
