| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human USP5 was used as the immunogen for the USP5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
USP5 Antibody / Ubiquitin specific peptidase 5 is a anti-USP5 Rabbit antibody Recombinant Rabbit Monoclonal clone 32U18 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: USP5
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32U18, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
USP5 antibody is commonly used in research on protein degradation, signal transduction, and cancer biology. By detecting USP5, scientists can study how ubiquitin processing enzymes control proteostasis and prevent accumulation of abnormal proteins. Dysregulation of USP5 activity leads to disruptions in protein quality control and contributes to disease.
In western blot assays, USP5 antibody detects protein bands corresponding to the enzyme in cell and tissue extracts. Immunohistochemistry reveals localization in cytoplasmic and nuclear compartments, while immunofluorescence highlights its presence in proteasome rich regions. These applications make USP5 antibody versatile for tracking ubiquitin protease activity.
USP5 has been implicated in cancer progression, where it regulates stability of tumor suppressors and oncogenes. Elevated USP5 expression supports tumor cell survival and proliferation by maintaining ubiquitin pools and stabilizing growth promoting proteins. By applying USP5 antibody, researchers can assess how deubiquitinating enzymes contribute to malignancy and therapy resistance.
Beyond cancer, USP5 plays roles in neurodegeneration, immune regulation, and viral infection. In neurons, altered USP5 activity contributes to proteotoxic stress and synaptic dysfunction. In immunity, it regulates signaling pathways dependent on ubiquitination, affecting cytokine responses and pathogen defense. This broad functional spectrum highlights the research value of USP5 antibody in multiple biological contexts.
USP5 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
