| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Caspase 9 recombinant protein (amino acids K18-Q417) was used as the immunogen for the Zebrafish Caspase 9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Caspase 9 Antibody / Casp9 is a anti-CASP9 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: CASP9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Following mitochondrial outer membrane permeabilization, cytochrome c is released into the cytosol, leading to the formation of the apoptosome complex. Caspase 9 is recruited and activated within this complex, initiating a proteolytic cascade that culminates in the activation of effector caspases such as Caspase 3 and Caspase 7. In zebrafish, casp9 is expressed in multiple tissues during development and is crucial for eliminating damaged, mis-specified, or excess cells.
Zebrafish Caspase 9 is widely used in research on cell death mechanisms, neurodevelopment, cancer biology, and toxicology, providing an in vivo model to study mitochondria-mediated apoptosis and the effects of pro- and anti-apoptotic signals. Due to its high degree of functional conservation with mammalian caspase-9, it serves as a valuable tool for both basic and translational research.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
