| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Cdc48 recombinant protein (amino acids D10-K512) was used as the immunogen for the Zebrafish Cdc48 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Cdc48 Antibody / Valosin-containing protein / Vcp is a anti-CDC48 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: CDC48
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Zebrafish Cdc48 is an ortholog of the human CDC48 protein, also known as p97. It shares significant sequence and functional conservation with its human counterpart, ensuring that it performs similar roles in regulating protein turnover, signaling pathways, and cell cycle progression. Like human p97, zebrafish Cdc48 is essential for the regulation of processes such as DNA repair, organelle biogenesis, and apoptosis, and its dysfunction can result in various diseases, including neurodegenerative disorders and certain cancers.
The use of a Zebrafish Cdc48 antibody is an invaluable tool for studying protein quality control mechanisms and their role in cellular stress responses. Such antibodies are essential for applications including western blot, immunohistochemistry, immunofluorescence, and ELISA. With a Zebrafish Cdc48 antibody, researchers can analyze Cdc48 expression in different tissues, study its involvement in proteostasis, and investigate how alterations in its function impact development and disease.
Currently, no isoforms of zebrafish Cdc48 have been documented, but its essential role in cellular maintenance and its conservation across species make it an important target for research. A high-quality Zebrafish Cdc48 antibody provides essential insights into cellular stress mechanisms and the regulation of the ubiquitin-proteasome system in zebrafish models.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
