| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Csf1r recombinant protein (amino acids D409-C977) was used as the immunogen for the Zebrafish Csf1r antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Csf1r Antibody / M-csfr is a anti-CSF1R Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cell membrane.
Key elements and design rationale
- Target: CSF1R
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
Csf1r is activated by binding to its primary ligand, colony stimulating factor one, which triggers intracellular signaling pathways involved in cell survival, proliferation, and differentiation. In zebrafish, Csf1r is expressed in the yolk sac and hematopoietic tissues early in development and marks cells that give rise to macrophages, including microglia in the brain and Kupffer cells in the liver.
Disruption of Csf1r function in zebrafish leads to reduced numbers or absence of tissue macrophages, providing a valuable model for studying the roles of these cells in development, immunity, and disease. Zebrafish Csf1r is widely used in research related to innate immunity, inflammation, neuroimmunology, and hematopoiesis.
Due to its conservation across vertebrate species, zebrafish Csf1r is also a powerful tool for investigating genetic and pharmacological regulation of macrophage biology and for modeling diseases such as neurodegeneration and immune deficiencies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
