| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish E-cadherin recombinant protein (amino acids Y23-D864) was used as the immunogen for the Zebrafish E-cadherin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish E-cadherin Antibody / Cdh1 / Cadherin 1 is a anti-CDH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cell membrane, cytoplasm.
Key elements and design rationale
- Target: CDH1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
E cadherin is a transmembrane glycoprotein that mediates homophilic cell to cell adhesion by forming adherens junctions between neighboring epithelial cells. It is a critical component of the cadherin catenin complex, which connects the actin cytoskeleton to the plasma membrane, thereby regulating tissue architecture and cell polarity.
In zebrafish embryos, E cadherin is expressed from the earliest stages of development and is especially important for coordinating cell movements and maintaining cohesion during morphogenesis. Loss or disruption of E cadherin function leads to severe defects in cell adhesion, tissue separation, and embryonic patterning.
Because of its conserved role in epithelial biology, zebrafish E cadherin is widely used in research focused on developmental biology, cancer metastasis, tissue regeneration, and epithelial to mesenchymal transition. It also serves as a valuable marker for epithelial cell identity and cell junction integrity in various model systems.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
