| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Estrogen receptor 2b recombinant protein (amino acids R27-N592) was used as the immunogen for the Zebrafish Estrogen receptor 2b antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Estrogen receptor 2b Antibody / Esr2b is a anti-2B Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: 2B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Esr2b is expressed in a variety of tissues including the brain, liver, gonads, and developing embryos. Upon binding to estrogens such as estradiol, Esr2b translocates to the nucleus where it binds to specific DNA sequences known as estrogen response elements to modulate gene transcription. Esr2b has been shown to influence processes such as sexual differentiation, oocyte maturation, and endocrine signaling.
Esr2b is functionally distinct from the closely related Esr2a and Esr1 receptors, and its expression pattern suggests it may have specialized roles in estrogen signaling pathways. Zebrafish provide a powerful model to study Esr2b function in vivo, including the effects of environmental estrogens and endocrine disrupting compounds on gene regulation and reproductive health.
Due to its importance in estrogen mediated gene regulation and its responsiveness to hormonal and environmental cues, zebrafish Esr2b is widely used in research focused on reproductive biology, developmental endocrinology, toxicology, and hormone responsive gene networks.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
