| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Etv4 recombinant protein (amino acids M1-E333) was used as the immunogen for the Zebrafish Etv4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Etv4 Antibody / ETS translocation variant 4 / Pea3 is a anti-ETV4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: ETV4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Etv4 functions as a key downstream effector of FGF signaling pathways, mediating transcriptional responses critical for tissue patterning and organogenesis. During zebrafish embryogenesis, etv4 is expressed in a variety of dynamic domains including the posterior mesoderm, developing somites, neural crest, and the pronephros. It acts in concert with other Pea3 subfamily members such as Etv5 to regulate genes involved in cell migration, proliferation, and differentiation.
Zebrafish etv4 has been extensively studied in the context of limb and fin development, neuromast formation, and branchial arch morphogenesis, making it a useful molecular marker for FGF activity and mesenchymal-epithelial interactions. Disruption of etv4 expression or function impairs normal developmental processes, underscoring its regulatory importance.
Due to its conserved function and regulated expression, zebrafish Etv4 serves as a valuable model for studying ETS transcription factors in vertebrate development and disease pathways, including cancer metastasis and skeletal disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
