| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Gfap recombinant protein (amino acids R123-P444) was used as the immunogen for the Zebrafish Gfap antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Gfap Antibody / Glial fibrillary acidic protein is a anti-Zebrafish Gfap Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Gfap
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Gfap is strongly expressed in the neural tube, spinal cord, and brain ventricles of developing zebrafish embryos. These radial glial cells give rise to neurons and glia, making Gfap expression an important indicator of neurogenesis. In the mature zebrafish brain, Gfap remains expressed in astroglia and is associated with neural maintenance, repair, and regeneration.
Because zebrafish have a high capacity for neural regeneration, Gfap is also commonly used to study injury responses and repair mechanisms in the brain and spinal cord. The conservation of Gfap structure and function across vertebrates makes zebrafish a valuable model for understanding the roles of glial cells in development, neurodegeneration, and central nervous system diseases.
Zebrafish Gfap is widely applied in developmental neuroscience, regenerative medicine, and glial biology research. It is also a standard marker in transgenic models used to trace glial lineages and study brain architecture.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
