| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Hbae1.1 recombinant protein (amino acids M1-R143) was used as the immunogen for the Zebrafish Hbae1.1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Hbae1.1 Antibody / Hemoglobin alpha embryonic 1.1 is a anti-Zebrafish Hbae1.1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: Zebrafish Hbae1.1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Hbae1.1 is expressed primarily in the intermediate cell mass, which is the site of primitive erythropoiesis in zebrafish embryos. It forms part of the embryonic hemoglobin complex that enables efficient oxygen delivery during rapid cell growth and organ formation. As development progresses, Hbae1.1 expression decreases while adult forms of hemoglobin become dominant.
Due to its specific and transient expression pattern, Hbae1.1 is widely used as a molecular marker for primitive erythroid cells and early blood development in zebrafish. It provides a valuable readout for studying hematopoiesis, gene regulation during red blood cell formation, and the effects of genetic mutations or chemical exposures on early blood cell differentiation.
Zebrafish Hbae1.1 is an important tool in developmental biology, hematology, toxicology, and genetic screening for blood related disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
