| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Hspa5 recombinant protein (amino acids D103-L650) was used as the immunogen for the Zebrafish Hspa5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Hspa5 Antibody / Grp78 / Bip is a anti-HSPA5 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm (ER).
Key elements and design rationale
- Target: HSPA5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Hspa5 facilitates proper protein folding, prevents aggregation of misfolded proteins, and targets irreversibly misfolded proteins for degradation. It is also a key regulator of ER homeostasis, acting as a sensor for misfolded proteins and an upstream effector in activating UPR pathways, including PERK, ATF6, and IRE1.<
In zebrafish, hspa5 is expressed ubiquitously during early embryogenesis and is upregulated in response to physiological and environmental stressors such as hypoxia, oxidative stress, and toxin exposure. Its expression is especially prominent in secretory tissues and rapidly developing organs such as the liver, pancreas, and brain.
Zebrafish Hspa5 serves as a useful marker for ER stress and has been widely used in models of developmental defects, neurodegeneration, metabolic disease, and toxicology screening. Due to its evolutionary conservation and functional parallels to mammalian GRP78, zebrafish Hspa5 is a valuable target for studying cellular stress responses and disease mechanisms in vivo.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
