| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Il-1 beta recombinant protein (amino acids M1-I284) was used as the immunogen for the Zebrafish Il-1 beta antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Il-1 beta Antibody / Il1b / Interleukin 1 is a anti-IL-1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: IL-1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
Il1b is primarily produced by macrophages and other immune cells following recognition of pathogens or cellular damage. Once activated, Il1b initiates a cascade of signaling events that result in the expression of additional cytokines, chemokines, and adhesion molecules, thereby amplifying the inflammatory response. In zebrafish, Il1b is expressed early during immune challenge and is a key marker of immune system activation.
Il1b is also involved in the regulation of hematopoiesis, fever, and wound healing. Its expression is tightly controlled at both the transcriptional and posttranslational levels to ensure a balanced immune response. Disruption or overactivation of Il1b can lead to excessive inflammation and tissue damage.
Due to its evolutionary conservation and well defined function, zebrafish Il1b is widely used in studies of inflammation, infection, immune system development, and inflammatory disease models. It serves as a valuable tool for drug screening and for investigating the molecular pathways that regulate cytokine signaling in vertebrates.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
