| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Mmp9 recombinant protein (amino acids D33-L680) was used as the immunogen for the Zebrafish Mmp9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Mmp9 Antibody / Matrix metalloproteinase 9 is a anti-MMP9 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, nuclear, secreted.
Key elements and design rationale
- Target: MMP9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
Mmp9 functions by degrading components of the extracellular matrix such as collagen and gelatin, allowing for tissue restructuring and cell migration. Its expression is tightly regulated and often induced by injury, infection, or developmental cues. In zebrafish, Mmp9 is notably upregulated in response to fin amputation and participates in the regeneration process by clearing damaged matrix and enabling cell proliferation.
In the immune system, zebrafish Mmp9 is expressed in neutrophils and macrophages and contributes to leukocyte recruitment and inflammation resolution. It is also involved in remodeling of the brain and other organs during development and in response to environmental stressors.
Due to its conservation across vertebrate species, zebrafish Mmp9 serves as a powerful model for studying matrix dynamics, regenerative biology, and inflammatory diseases. It is commonly used in genetic and chemical screens to explore pathways related to tissue remodeling and immune function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
