| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Nkx2.2a recombinant protein (amino acids M1-W269) was used as the immunogen for the Zebrafish Nkx2.2a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Nkx2.2a Antibody / Nkx2.2 is a anti-Zebrafish Nkx2.2a Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Nkx2.2a
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Nkx2.2a is one of two paralogs, the other being Nkx2.2b, resulting from a genome duplication event in teleosts. Nkx2.2a is considered a functional ortholog of the human NKX2 dash 2 gene. Both proteins are highly conserved and share similar developmental roles, particularly in the specification of ventral neural progenitors and in endocrine pancreas formation.
In zebrafish embryos, Nkx2.2a is expressed in the ventral neural tube, including the regions that give rise to motor neurons, oligodendrocyte precursors, and serotonergic neurons. It acts downstream of sonic hedgehog signaling and is essential for defining the identity of specific neuronal subtypes. Nkx2.2a also contributes to the early development of the pancreas, where it helps regulate differentiation of insulin producing beta cells and other endocrine cell types.
Loss of Nkx2.2a function in zebrafish leads to defects in neuronal patterning and impaired endocrine pancreas development, mirroring the phenotypes observed in mammalian systems. Due to its conservation with the human NKX2 dash 2 gene and its involvement in key developmental processes, zebrafish Nkx2.2a is widely used as a model to study neurogenesis, pancreas biology, and transcriptional regulation in vertebrates.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
