| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Nploc4 recombinant protein (amino acids M1-C510) was used as the immunogen for the Zebrafish Nploc4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Nploc4 Antibody / Npl4 / Nuclear protein localization protein 4 is a anti-NPLOC4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: NPLOC4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Nploc4 is expressed in multiple tissues and is especially active in proliferative or metabolically active cells. The zebrafish Nploc4 protein is an ortholog of the human NPLOC4 gene. Both versions of the protein share strong sequence and functional conservation, indicating that zebrafish can serve as a reliable model for studying NPLOC4 related biology.
Functionally, Nploc4 is part of a complex with Ufd1 and p97, and this complex is critical for endoplasmic reticulum associated degradation, chromatin remodeling, and cell cycle regulation. Disruption of Nploc4 function in zebrafish leads to defects in protein homeostasis and developmental abnormalities, especially in systems that are sensitive to proteotoxic stress such as the nervous system and musculature.
Given its central role in maintaining cellular protein quality control and its conservation with human NPLOC4, zebrafish Nploc4 is commonly used in developmental studies and disease models related to protein aggregation, neurodegeneration, and cellular stress adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
