| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Tumor protein 53 (amino acids D29-Q358) was used as the immunogen for the Zebrafish p53 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish p53 Antibody / Tp53 / Tumor protein 53 is a anti-P53 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: P53
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, the p53 protein is an ortholog of the human TP53 gene, and both share highly conserved structural domains, including the DNA binding domain and the oligomerization domain, which are crucial for its function as a transcription factor. The zebrafish p53 protein shares many functional similarities with its human counterpart, including its ability to activate target genes like p21 and Bax in response to cellular stress and its involvement in apoptosis regulation. This makes zebrafish a valuable model for studying the role of p53 in cancer biology, DNA damage responses, and developmental processes.
During zebrafish development, p53 is involved in embryogenesis, cell differentiation, and tissue remodeling. p53’s function is particularly important in the early stages of zebrafish development, where it helps maintain genomic stability by responding to various stress signals. In cases of severe DNA damage or other cellular insults, p53 activation can lead to cell cycle arrest or apoptosis, which ensures that damaged cells do not proliferate, thereby preventing potential tumor formation.
The zebrafish model has been widely used in cancer research due to the ease of genetic manipulation and the similarity of key signaling pathways like the p53 pathway. p53 mutations in zebrafish, like in humans, can lead to abnormal cellular proliferation, tissue development defects, and tumor formation. Understanding the molecular pathways controlled by p53 in zebrafish is crucial for modeling human diseases such as cancer, neurodegeneration, and aging.
Given its evolutionary conservation and critical role in cell cycle regulation, apoptosis, and tumor suppression, zebrafish p53 is a powerful tool for studying oncogenesis, genomic stability, drug screening, and genetic disorders in vertebrate models.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
