| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of zebrafish Pabpc4 protein was used as the immunogen for the Pabpc4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pabpc4 Antibody / Polyadenylate-binding protein is a anti-PABPC4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PABPC4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Pabpc4 is an ortholog of the human PABPC4 gene. Both zebrafish and human Pabpc4 share significant sequence similarity and conserved functional domains, particularly in the RNA-binding regions. This high level of conservation between zebrafish and human Pabpc4 makes the zebrafish protein an excellent model for studying RNA regulation and its impact on cellular processes like development, cell differentiation, and stress response.
The primary function of Pabpc4 in zebrafish is to bind to the poly(A) tail of mRNA, which is a key feature in regulating mRNA stability and translation initiation. Pabpc4 facilitates the recruitment of translation initiation factors to the mRNA and plays a role in translational control, particularly in response to environmental stressors or changes in nutrient availability. Pabpc4 also participates in mRNA decay pathways, ensuring that mRNAs are properly degraded when they are no longer needed, thus maintaining cellular homeostasis.
Zebrafish Pabpc4 is expressed in multiple tissues, including the brain, muscle, and embryonic tissues, and is particularly important during early development. It regulates mRNA translation and is crucial for protein synthesis during the rapid cell divisions and differentiation events that occur during embryogenesis.
Given its role in RNA metabolism and the regulation of gene expression, zebrafish Pabpc4 is a valuable model for studying molecular biology, genetic regulation, and developmental processes. The conservation of its function across species also makes it relevant for research into human diseases related to RNA processing and gene regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
