| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pard3aa recombinant protein (amino acids M1-Q1303) was used as the immunogen for the Zebrafish Pard3aa antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pard3aa Antibody / Partitioning defective 3 is a anti-PARD3AA Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm, cell membrane, cell junctions (human similarity).
Key elements and design rationale
- Target: PARD3AA
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Pard3aa is an ortholog of the human PARD3 gene. Both zebrafish and human Pard3aa share high sequence similarity and functional conservation, particularly in the regulation of cell polarity and cellular architecture. The similarities between the zebrafish and human versions of Pard3aa make zebrafish an excellent model for studying the molecular mechanisms of cell polarity, embryonic development, and tissue morphogenesis.
Pard3aa plays a vital role in the development of epithelial tissues, including the brain, gut, and heart, where it helps to establish and maintain apical-basal polarity. During embryogenesis, Pard3aa is involved in controlling embryo axis formation, cell migration, and the establishment of cellular asymmetry. It regulates the distribution of key proteins involved in junction formation and membrane trafficking, ensuring proper cell-cell adhesion and tissue integrity.
Given its role in developmental biology, cellular architecture, and tissue polarity, zebrafish Pard3aa serves as a useful model for studying fundamental processes like neural development, organogenesis, and cell polarity diseases. It is also an important protein for investigating human conditions related to developmental disorders, epithelial cancers, and neurodegenerative diseases that involve defects in cell polarity and cellular organization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
