| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pcna recombinant protein (amino acids M1-S260) was used as the immunogen for the Zebrafish Pcna antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pcna Antibody / Proliferating cell nuclear antigen is a anti-Zebrafish Pcna Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Pcna
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Pcna is an ortholog of the human PCNA gene. Both zebrafish and human Pcna proteins are highly conserved in terms of their sequence and function, with both being involved in regulating DNA replication and cell cycle progression. The zebrafish Pcna protein shares the same functional domains as the human protein, including the sliding clamp domain, which is essential for its role in DNA polymerase stabilization and in facilitating replication fork progression.
Zebrafish Pcna is expressed in a variety of tissues, with prominent expression observed during embryonic development in rapidly proliferating cells. It is especially important in mitotic cells, where it helps facilitate efficient DNA replication during the S-phase of the cell cycle. In addition, Pcna participates in DNA repair pathways, including base excision repair and nucleotide excision repair, ensuring the correction of any replication errors or DNA damage caused by environmental stress.
The function of Pcna in zebrafish has been extensively studied, especially due to its role in development and tissue regeneration. During embryogenesis, Pcna is highly expressed in the neural tissue, heart, and muscle, which require constant cell division and DNA replication. Furthermore, Pcna is involved in the response to DNA damage and in the regulation of cell cycle checkpoints, especially in tissues undergoing rapid growth and development.
Given its central role in cellular proliferation, DNA repair, and genome stability, zebrafish Pcna is an essential protein for studying cell cycle regulation, DNA replication, mutagenesis, and genomic integrity. The high degree of conservation between zebrafish and human Pcna makes it an ideal model for investigating human diseases associated with cell cycle dysregulation, such as cancer and genetic disorders related to DNA repair.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
