| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pgam1 recombinant protein (amino acids Q43-K254) was used as the immunogen for the Zebrafish Pgam1 antibody. This antibody will detect the a & b isoforms. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pgam1 Antibody / Pgam1a / Pgam1b / Phosphoglycerate mutase is a anti-PGAM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PGAM1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Pgam1 is an ortholog of the human PGAM1 gene. Both zebrafish and human Pgam1 proteins share significant sequence similarity and perform equivalent functions in glycolytic metabolism. The protein in both species is involved in the reversible conversion of 3-phosphoglycerate to 2-phosphoglycerate, ensuring the smooth progression of glycolysis. Zebrafish Pgam1 is widely expressed in tissues that rely on high levels of metabolic activity, including the muscle, brain, and liver.
Zebrafish Pgam1 has isoforms, as is common for metabolic enzymes involved in energy metabolism. These isoforms may differ in their tissue distribution or functional properties, which is important for the regulation of glycolysis under different physiological conditions. These isoforms may be involved in tissue-specific functions and are essential for maintaining glycolytic flux in response to varying energy demands during development and growth.
In zebrafish, Pgam1 plays a critical role during embryogenesis, where metabolic regulation is essential for proper growth and tissue differentiation. It is also important for muscle development and neural tissue formation, where metabolic demands are high. Pgam1 is expressed in developing tissues, particularly those undergoing rapid cell division and differentiation.
Given its role in metabolism, cellular energy regulation, and development, zebrafish Pgam1 is an important protein for studying glycolysis, energy metabolism, and diseases related to metabolic dysfunction. It is particularly valuable in studies of muscle metabolism, neurodevelopment, and embryonic development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
