| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Phyhipl recombinant protein (amino acids M1-D337) was used as the immunogen for the Zebrafish Phyhipl antibody. This antibody will detect Phyhipl protein as well as the Phyhiplb isoform. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Phyhipl Antibody / Phyhiplb / Phytanoyl-CoA hydroxylase-interacting protein-like is a anti-Zebrafish Phyhipl Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: Zebrafish Phyhipl
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Phyhipl is an ortholog of the human PHYHIPL gene. Both zebrafish and human Phyhipl share high sequence similarity, particularly in the PH domain and IF2-like domain, suggesting conservation of function across species. This conservation makes zebrafish an excellent model for studying the molecular functions of Phyhipl and its involvement in cellular signaling and developmental processes.
Phyhipl is thought to play a role in the regulation of cellular stress responses, especially under conditions of oxidative stress or hypoxia, by modulating cellular signaling networks. It may also interact with cellular scaffolding proteins and signal transduction pathways, helping to coordinate responses to environmental and internal cellular stresses. This protein is likely involved in neural development, embryonic development, and organogenesis, particularly in the brain, heart, and muscle, where regulation of cell growth and differentiation is crucial.
Zebrafish Phyhipl has isoforms, which may vary in their domain structure, tissue-specific expression, and functional capacity. The isoforms could be differentially expressed in various tissues, reflecting the need for fine-tuned regulation in response to changing environmental and metabolic conditions. Isoform variation is likely important for tissue-specific functions during development and tissue regeneration.
Given its essential role in signal transduction, cell stress responses, and developmental regulation, zebrafish Phyhipl is an important model for investigating the molecular mechanisms underlying cellular stress responses, neurodevelopmental diseases, and cancer. Its functional conservation with the human protein makes it a valuable tool for studying human diseases that involve cellular signaling and developmental defects.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
