| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pi4kb recombinant protein (amino acids D394-D690) was used as the immunogen for the Zebrafish Pi4kb antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pi4kb Antibody / Phosphatidylinositol 4-kinase beta is a anti-PI4KB Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm (ER, Mitochondria).
Key elements and design rationale
- Target: PI4KB
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Pi4kb is an ortholog of the human PI4KB gene. Both zebrafish and human Pi4kb share substantial sequence conservation and functional similarities, particularly in their roles in lipid signaling and membrane trafficking. The high degree of conservation between species makes zebrafish an ideal model for studying the molecular function of Pi4kb in cellular signaling, membrane dynamics, and vesicular trafficking.
Zebrafish Pi4kb is highly expressed in tissues with high demands for membrane turnover and vesicular trafficking, such as the brain, heart, and muscle. The protein plays a significant role in the formation and function of membrane-bound organelles, including endosomes, lysosomes, and the Golgi apparatus. It also regulates the recycling of phosphoinositides, which are essential for maintaining the integrity of cellular membranes and signal transduction pathways.
The Pi4kb gene in zebrafish has isoforms, which may differ in their tissue distribution and functional roles. These isoforms are thought to be important for the fine-tuned regulation of lipid signaling in different cell types and under varying physiological conditions. Isoform variation is likely linked to developmental stages and differentiation processes, particularly in tissues that require rapid changes in membrane composition.
Given its essential role in membrane dynamics, lipid signaling, and vesicular trafficking, zebrafish Pi4kb is an important model for studying diseases related to membrane dysfunction, neurological disorders, and metabolic diseases. Its role in regulating phosphoinositide metabolism also makes it a valuable target for research into diseases such as cancer, cardiovascular disorders, and neurodegenerative diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
