| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Polr2b recombinant protein (amino acids E49-Q1145) was used as the immunogen for the Zebrafish Polr2b antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Polr2b Antibody / DNA-directed RNA polymerase subunit beta is a anti-POLR2B Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: POLR2B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Polr2b is an ortholog of the human POLR2B gene. The zebrafish and human Polr2b proteins exhibit significant sequence conservation, reflecting the evolutionary conservation of their functions in RNA transcription. This high degree of homology makes zebrafish an excellent model for studying the role of Polr2b in gene expression and transcription regulation across species.
Polr2b is ubiquitously expressed in zebrafish tissues, with particularly high expression levels in tissues such as the brain, heart, and muscle. These tissues rely heavily on the proper regulation of gene expression during development and cellular differentiation. Polr2b is essential for the transcription of protein-coding genes during early embryogenesis, as well as throughout the life cycle of the organism, playing a key role in the regulation of cellular growth, differentiation, and organ development.
Zebrafish Polr2b has isoforms, which may vary in their functional properties and tissue-specific expression. These isoforms could be involved in the fine-tuned regulation of transcription during different developmental stages or in response to environmental cues. Isoform diversity in Polr2b allows the protein to meet the specific transcriptional needs of different cell types, especially in tissues undergoing rapid differentiation or experiencing specific environmental stressors.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
