| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Ppp3r1 recombinant protein (amino acids E53-V175) was used as the immunogen for the Zebrafish Ppp3r1 antibody. This antibody will detect both the a and b isoform. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Ppp3r1 Antibody / Ppp3r1a / Ppp3r1b is a anti-PPP3R1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PPP3R1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Ppp3r1 is an ortholog of the human PPP3R1 gene. The zebrafish and human versions of Ppp3r1 exhibit significant sequence conservation, especially in regions that are essential for interaction with calcineurin. This conservation suggests that Ppp3r1 in zebrafish performs similar regulatory functions as its human counterpart in calcium-mediated signaling and cellular processes. The zebrafish model is particularly useful for studying calcium signaling, immune regulation, and neural and cardiac development.
Zebrafish Ppp3r1 may also have isoforms, which are generated through alternative splicing. These isoforms could exhibit tissue-specific expression and play distinct roles in regulating calcineurin activity in different physiological conditions or during developmental stages. Isoform diversity may allow for fine-tuned regulation of calcineurin signaling in response to various cellular and environmental cues.
In zebrafish, Ppp3r1 is widely expressed in neural tissues, cardiac cells, and immune cells, reflecting its essential roles in neural development, immune responses, and cardiac function. Its involvement in calcineurin activation plays a pivotal role in regulating gene expression and cell differentiation. Disruption of Ppp3r1 or calcineurin signaling in zebrafish can lead to defects in neural patterning, immune response modulation, and cardiac development, providing a valuable model for studying diseases related to abnormal calcium signaling, such as cardiovascular diseases, neurodegenerative disorders, and autoimmune diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
