| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Prdx4 recombinant protein (amino acids S167-N260) was used as the immunogen for the Zebrafish Prdx4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Prdx4 Antibody / Peroxiredoxin 4 is a anti-PRDX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PRDX4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Prdx4 is an ortholog of the human PRDX4 gene, with both proteins sharing a high degree of sequence conservation in their peroxiredoxin domain, which is essential for their antioxidant activity. This functional conservation suggests that Prdx4 in zebrafish performs similar protective roles as its human counterpart in oxidative stress response and cellular homeostasis. The zebrafish model, therefore, offers an excellent system to study the roles of peroxiredoxins in cell survival, protein quality control, and diseases related to oxidative damage.
Prdx4 in zebrafish also has isoforms, which may be generated through alternative splicing. These isoforms can exhibit tissue-specific expression patterns and may have distinct functions depending on the cellular environment or developmental stage. The existence of isoforms allows for more precise regulation of redox balance in response to physiological stress and disease conditions, including conditions involving chronic inflammation or neurodegenerative disorders.
Zebrafish Prdx4 is expressed in various tissues, including the liver, brain, muscles, and heart, where it plays an essential role in protecting cells from oxidative damage and maintaining cellular integrity. Its expression is especially important in the endoplasmic reticulum, where it participates in the oxidative folding of proteins. Prdx4 also contributes to the regulation of immune responses by modulating inflammatory signaling pathways, making it essential for maintaining immune homeostasis and protecting tissues from inflammatory damage.
Given its role in antioxidant defense, cell survival, and immune regulation, zebrafish Prdx4 is an important protein for studying oxidative stress, neurodegenerative diseases, cardiovascular health, and immune system function. Disruptions in Prdx4 function in zebrafish can lead to increased cellular oxidative damage, impaired protein folding, and inflammatory responses, making it a valuable model for investigating diseases related to oxidative stress and cellular dysfunction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
