| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmd2 recombinant protein (amino acids S209-A897) was used as the immunogen for the Zebrafish Psmd2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmd2 Antibody / 26S proteasome non-ATPase regulatory subunit 2 is a anti-PSMD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMD2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Psmd2 is the ortholog of the human PSMD2 gene, showing significant sequence similarity and functional conservation between the two species. The zebrafish Psmd2 protein shares essential domains responsible for interaction with other proteasomal subunits and regulatory factors, enabling the proper assembly and function of the proteasome complex.
Psmd2 in zebrafish may also have isoforms resulting from alternative splicing events. These isoforms may differ in their tissue-specific expression or regulatory roles, potentially affecting proteasomal activity in distinct developmental stages or cellular contexts. The diversity of these isoforms allows for fine-tuned regulation of protein degradation in response to various cellular stresses, such as oxidative stress or DNA damage.
In zebrafish, Psmd2 is expressed in a wide range of tissues, particularly in those with high cell turnover and protein synthesis, such as the brain, liver, and kidney. This pattern of expression reflects the essential role of Psmd2 in maintaining protein quality control, regulating the degradation of misfolded or damaged proteins, and modulating signaling pathways critical for cellular functions.
The zebrafish model, with its conserved proteasomal components, is valuable for studying proteasome-related diseases in humans. Disruptions in PSMD2 function are associated with various human disorders, including neurodegenerative diseases and cancers, where defective protein degradation leads to the accumulation of abnormal proteins. Zebrafish Psmd2 serves as an excellent model for understanding the mechanisms of proteasomal dysfunction and for screening potential therapies targeting the proteasome.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
