| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmd4 recombinant protein (amino acids M1-D322) was used as the immunogen for the Zebrafish Psmd4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmd4 Antibody / Psmd4a / Psmd4b / 26S proteasome non-ATPase regulatory subunit 4 is a anti-PSMD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PSMD4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Psmd4 is the ortholog of the human PSMD4 gene, showing a high degree of sequence identity and functional conservation. Both the zebrafish and human proteins share the same basic structural motifs essential for proteasomal function, including interaction domains that allow for proper assembly and regulation of the proteasomal complex.
Zebrafish Psmd4 may have isoforms resulting from alternative splicing events, although specific isoform variants may not be fully characterized. These isoforms could potentially exhibit tissue-specific expression or alternative regulatory roles, further contributing to the fine-tuned regulation of proteasomal activity. The functional diversity provided by these isoforms allows for specialized roles in different tissues or developmental stages, ensuring efficient protein degradation and homeostasis under varying conditions.
The expression of Psmd4 in zebrafish is widespread, with notable expression in tissues that undergo rapid cell division or have high protein turnover, such as the brain, liver, and kidney. This expression profile highlights the critical role of Psmd4 in maintaining protein integrity, regulating cellular stress responses, and ensuring proper protein homeostasis, particularly in rapidly growing or dividing cells.
Given the functional conservation between zebrafish and human Psmd4, zebrafish serves as an ideal model for studying the molecular mechanisms underlying proteasomal dysfunction in humans. In particular, PSMD4 mutations in humans are associated with certain diseases, including cancer and neurodegenerative disorders, where impaired proteasomal degradation leads to the accumulation of misfolded or damaged proteins. Zebrafish Psmd4 is a valuable tool for investigating proteasome-related diseases and for screening potential therapeutic agents that can modulate proteasomal activity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
