| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmd7 recombinant protein (amino acids M1-N274) was used as the immunogen for the Zebrafish Psmd7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmd7 Antibody / 26S proteasome non-ATPase regulatory subunit 7 is a anti-PSMD7 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PSMD7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Psmd7 is the ortholog of the human PSMD7 gene, sharing a high degree of sequence similarity and functional conservation. The zebrafish and human versions of Psmd7 contain similar domains that allow interaction with other proteasomal subunits, enabling the proper assembly and function of the 26S proteasome complex. This functional conservation underscores the relevance of zebrafish as a model organism for studying proteasomal processes and their role in human health.
Psmd7 in zebrafish may have isoforms resulting from alternative splicing, although specific isoforms may not be fully characterized. These isoforms could play roles in different tissues or developmental stages, contributing to the fine-tuning of proteasomal activity. Isoform variation could also provide differential regulation of proteasome function in response to environmental stress, cellular signaling, or developmental needs.
In zebrafish, Psmd7 is expressed in a variety of tissues, especially those with high protein turnover, such as the brain, liver, and kidney. This expression pattern reflects the protein's essential role in controlling protein degradation, supporting cellular functions such as stress response, apoptosis, and differentiation.
Given the high conservation between zebrafish Psmd7 and human PSMD7, the zebrafish model is valuable for studying proteasomal dysfunctions that contribute to diseases in humans, such as cancer, neurodegenerative disorders, and immune dysfunctions. Disruption in PSMD7 has been linked to altered proteasomal activity, which can lead to the accumulation of damaged proteins and cellular stress. The zebrafish model can be used to explore potential therapeutic approaches aimed at modulating proteasomal function and preventing the pathological effects associated with these diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
