| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Rab1a recombinant protein (amino acids R71-T195) was used as the immunogen for the Zebrafish Rab1a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Rab1a Antibody / Rab1aa / Rab1ab is a anti-RAB1A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: RAB1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Rab1a is the ortholog of the human RAB1A gene, with a high degree of sequence conservation and functional similarity. Both the zebrafish and human versions of Rab1a share conserved GTP-binding domains and the mechanisms necessary for vesicle trafficking. The role of Rab1a in zebrafish mirrors its function in humans, supporting processes such as protein secretion, cellular homeostasis, and membrane transport. This functional conservation makes zebrafish an excellent model for studying vesicular trafficking mechanisms and associated diseases.
In zebrafish, Rab1a exists as Rab1aa and Rab1ab, which are paralogous genes resulting from gene duplication events. Both Rab1aa and Rab1ab encode proteins with similar functions but may exhibit tissue-specific expression patterns or slight differences in their regulatory roles. While Rab1aa and Rab1ab share high sequence homology, they might have distinct roles during development or in various physiological contexts. Isoforms of Rab1a could arise through alternative splicing, allowing for specialized functions in different tissues or developmental stages.
The Rab1a proteins, including Rab1aa and Rab1ab, play significant roles in cellular processes like vesicle formation, transport, and fusion, especially in tissues with active secretion and protein processing, such as the liver, pancreas, and neurons. These proteins are involved in maintaining the proper function of the ER and Golgi, essential for protein trafficking and cellular signaling. Their roles are particularly important in maintaining the balance of protein homeostasis in cells.
Given the high conservation between zebrafish Rab1a (including Rab1aa and Rab1ab) and human RAB1A, zebrafish serve as an ideal model for investigating the molecular basis of diseases related to defects in vesicle trafficking, such as neurodegenerative diseases, metabolic disorders, and certain types of cancer. By studying Rab1a and its paralogs, researchers can explore therapeutic strategies aimed at modulating vesicle trafficking pathways, which could help in the treatment of these diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
