| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Rab4a recombinant protein (amino acids D105-C213) was used as the immunogen for the Zebrafish Rab4a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Rab4a Antibody / Rab-4A is a anti-RAB4A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: RAB4A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Rab4a is the ortholog of the human RAB4A gene, sharing a high degree of sequence conservation and performing similar functions in vesicular trafficking and endosome regulation. Both zebrafish Rab4a and human RAB4A are involved in the recycling of membrane receptors and other cargo, which is critical for processes like nutrient uptake, receptor signaling, and maintaining cell surface composition.
While Rab4a in zebrafish is well-conserved with its human counterpart, it may exhibit isoforms due to alternative splicing. These isoforms, though not fully characterized, could potentially have tissue-specific expression or variations in functionality based on different cellular contexts or developmental stages. The presence of isoforms of Rab4a could provide a mechanism for fine-tuning the regulation of endosome recycling in different tissues or under various physiological conditions.
Rab4a is expressed in tissues that rely heavily on endosomal recycling, such as the liver, kidney, and neurons, where its function in trafficking and receptor recycling plays an important role in cellular homeostasis and communication. In particular, Rab4a is critical for maintaining the flow of membrane components and proteins to ensure normal cellular signaling, nutrient uptake, and overall cell function.
Zebrafish Rab4a, due to its high degree of conservation with human RAB4A, is an excellent model for studying endocytic pathways, vesicular trafficking, and diseases associated with defective membrane recycling. Disorders related to abnormal Rab4 function have been linked to defects in endocytosis and cellular signaling, including neurodegenerative diseases, cancer, and metabolic disorders. Studying Rab4a in zebrafish offers valuable insights into these diseases and could aid in the development of therapeutic strategies targeting Rab GTPase pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
