| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Sdha recombinant protein (amino acids D132-R551) was used as the immunogen for the Zebrafish Sdha antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Sdha Antibody / Succinate dehydrogenase A is a anti-Zebrafish Sdha Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Sdha
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Sdha is an ortholog of the human SDHA protein and shares a high degree of sequence and functional conservation. In both species, Sdha functions as the catalytic subunit of succinate dehydrogenase and is required for efficient ATP generation. Mutations or disruptions in Sdha can impair mitochondrial function, leading to metabolic disorders and contributing to pathologies such as cancer and neurodegeneration.
There is no evidence of multiple isoforms of zebrafish Sdha, and its structure and activity are highly conserved across vertebrates. The zebrafish model provides a valuable system for studying mitochondrial metabolism and diseases related to complex II dysfunction due to the evolutionary similarity to human SDHA.
Antibodies against zebrafish Sdha protein are widely used as mitochondrial markers and for studying metabolic pathways. These antibodies are suitable for applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA, enabling the investigation of mitochondrial dynamics and energy metabolism in zebrafish tissues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
